Administration of three natural products as protective agents against the genotoxic and cytotoxic effect of methotrexate in mice [mus musculus]

Methotrexate is an antimetabolite and antifolate drug. It is used in treatment of cancer, autoimmune diseases. 210 healthy adult male Swiss albino mice [Mus musculus], were allotted among three groups. The animal were given daily [0.065mg/ml] of methotrexate intraperitonealy followed by injection of Omega-3 plus at dose [8.58 mg/20g mice], Apple at dose [1.43 mg/20 g mice] and Psyllium at dose [18.2mg /20 g mice] for five days. Various Structural and numerical chromosomal aberrations in bone marrow cells; mitotic activity and sperm head abnormality were recorded, quantitated, and statistically analyzed. Also DNA extraction and apoptosis detection in liver was done. The intensity of apoptotic bands located at 200 b p; 400b p ; 600 b p;800 b p and intact DNA measured by software Gel Pro program as maximum optical density values. Methotrexate had adverse effect on chromosomal and sperm head structure, also it induce apoptosis, necrosis and decrease total DNA in mice liver.Omega-3 plus, Apple, and Psyllium attenuate the methotrexate-related toxic effects

Some abnormal maternal behaviours and their relation with chromosomal aberration in rabbits

Forty doe rabbits of both breeds [New Zeal and Native] were used through consecutive three parities to investigate the possibility of the effect of chromosomal aberrations on some abnormal maternal behavior [failure of the doe to build a maternal nest on the day of or prior to delivery, scattering and cannibalism]. The results revealed that there was a significant increase in the mean number of cells with structural aberrations in all does having abnormal maternal behavior with the exception of end to end association and centrometric attenuation in the native does. The numerical aberrations showed a significant effect only at two types of aberrations [monosomic and trisomic] in the New Zeal and does and also in polyploidy only in the native ones. Also, the results revealed that there was a significant increase in values of mitotic activity in does showing abnormal maternal behavior than those having normal maternal behavior. The results indicated that, the New Zeal and does which show abnormal maternal behavior have more genetic disorders than those of the native ones